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dual vector split intein base editor aav genome plasmids  (Addgene inc)


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    Addgene inc dual vector split intein base editor aav genome plasmids
    Dual Vector Split Intein Base Editor Aav Genome Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dual+vector+split+intein+base+editor+aav+genome+plasmids/Cbh_v5+AAV-CBE+C-terminal+(Plasmid+%23137176)/pm41381416-201-5-12
    Average 93 stars, based on 9 article reviews
    dual vector split intein base editor aav genome plasmids - by Bioz Stars, 2026-10
    93/100 stars

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    Clone Assay:

    Article Title: Efficient in vivo genome editing prevents hypertrophic cardiomyopathy in mice
    Article Snippet: .. The ABE8e-SpCas9-NG base editor (abbreviated in the text as ABE8e) was cloned into established dual-vector split-intein base editor AAV genome plasmids (Addgene plasmids 137176 and 137177) as described , . .. However, we replaced the ubiquitously expressing synthetic Cbh promoter with the cardiomyocyte-specific chicken cardiac troponin T promoter ( Tnnt2 ) , encoded on a ~800-bp oligonucleotide (gBlock, Integrated DNA Technologies) and XbaI/AgeI-digested AAV9 plasmids.

    Article Title: In vivo base editing rescues ADPKD in a humanized mouse model
    Article Snippet: .. The ABE9 was cloned into dual-vector split-intein base editor AAV genome plasmids (Addgene plasmids 137176 and 137177) as described in ref. . .. In brief, the ABE9 fragments were amplified by PCR, and then cloned into the N-AAV vector using isothermal assembly in NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs (NEB), E2621S).

    Article Title: In vivo base editing rescues ADPKD in a humanized mouse model.
    Article Snippet: .. The ABE9 was cloned into dual-vector split-intein base editor AAV genome plasmids (Addgene plasmids 137176 and 137177) as described in ref. 8. .. In brief, the ABE9 fragments were amplified by PCR, and then cloned into the N-AAV vector using isothermal assembly in NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs (NEB), E2621S).

    Article Title: Efficient in vivo genome editing prevents hypertrophic cardiomyopathy in mice.
    Article Snippet: .. The ABE8e-SpCas9-NG base editor (abbreviated in the text as ABE8e) was cloned into established dual-vector split-intein base editor AAV genome plasmids (Addgene plasmids 137176 and 137177) as described17,49. .. However, we replaced the ubiquitously expressing synthetic Cbh promoter with the cardiomyocyte-specific chicken cardiac troponin T promoter (Tnnt2)14, encoded on a ~800-bp oligonucleotide (gBlock, Integrated DNA Technologies) and XbaI/AgeI-digested AAV9 plasmids.

    Bioprocessing:

    Article Title: Efficient in vivo genome editing prevents hypertrophic cardiomyopathy in mice
    Article Snippet: .. The ABE8e-SpCas9-NG base editor (abbreviated in the text as ABE8e) was cloned into established dual-vector split-intein base editor AAV genome plasmids (Addgene plasmids 137176 and 137177) as described , . .. However, we replaced the ubiquitously expressing synthetic Cbh promoter with the cardiomyocyte-specific chicken cardiac troponin T promoter ( Tnnt2 ) , encoded on a ~800-bp oligonucleotide (gBlock, Integrated DNA Technologies) and XbaI/AgeI-digested AAV9 plasmids.

    Article Title: In vivo base editing rescues ADPKD in a humanized mouse model
    Article Snippet: .. The ABE9 was cloned into dual-vector split-intein base editor AAV genome plasmids (Addgene plasmids 137176 and 137177) as described in ref. . .. In brief, the ABE9 fragments were amplified by PCR, and then cloned into the N-AAV vector using isothermal assembly in NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs (NEB), E2621S).

    Article Title: In vivo base editing rescues ADPKD in a humanized mouse model.
    Article Snippet: .. The ABE9 was cloned into dual-vector split-intein base editor AAV genome plasmids (Addgene plasmids 137176 and 137177) as described in ref. 8. .. In brief, the ABE9 fragments were amplified by PCR, and then cloned into the N-AAV vector using isothermal assembly in NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs (NEB), E2621S).

    Article Title: Efficient in vivo genome editing prevents hypertrophic cardiomyopathy in mice.
    Article Snippet: .. The ABE8e-SpCas9-NG base editor (abbreviated in the text as ABE8e) was cloned into established dual-vector split-intein base editor AAV genome plasmids (Addgene plasmids 137176 and 137177) as described17,49. .. However, we replaced the ubiquitously expressing synthetic Cbh promoter with the cardiomyocyte-specific chicken cardiac troponin T promoter (Tnnt2)14, encoded on a ~800-bp oligonucleotide (gBlock, Integrated DNA Technologies) and XbaI/AgeI-digested AAV9 plasmids.



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    dual vector split intein base editor aav genome plasmids  (Addgene inc)
    93
    Addgene inc dual vector split intein base editor aav genome plasmids
    Dual Vector Split Intein Base Editor Aav Genome Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dual+vector+split+intein+base+editor+aav+genome+plasmids/Cbh_v5+AAV-CBE+C-terminal+(Plasmid+%23137176)/pm41381416-201-5-12
    Average 93 stars, based on 1 article reviews
    dual vector split intein base editor aav genome plasmids - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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